Co-extraction of DNA and PLFA from soil samples.
نویسندگان
چکیده
Lipid/DNA co-extraction from one sample is attractive in limiting biases associated with microbial community analysis from separate extractions. We sought to enhance established co-extraction methods and use high-throughput 16S rRNA sequencing to identify preferentially extracted taxa from co-extracted DNA. Co-extraction results in low DNA yields and distinct community structure changes.
منابع مشابه
A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
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ورودعنوان ژورنال:
- Journal of microbiological methods
دوره 115 شماره
صفحات -
تاریخ انتشار 2015